High performance liquid chromatographic analysis of MS23 piperidine analog MSP001 in rat plasma
MSP001 is a newly synthesized piperidine analog of the lead antihypertensive compound MS23 that dually targets cAMP-specific type 4 phosphodiesterase and L-type calcium channels. We validated an analytical protocol for MSP001 in rat plasma using high performance liquid chromatographic method. A C18 column and a phosphate/acetonitrile buffer were used to perform the chromatographic separation. UV detection was carried out at 307 nm, a wavelength at which an absorption peak was detected for this group of compounds. The calibration curve for MSP001 was linear in the range from 25 to 10,000 ng/ml. The limit of quantification (LOQ) was 25 ng/ml. The results demonstrate that this method has high linearity (R = 0.99995), compound specificity, and acceptable precision/accuracy. The protocol is suitable for in vivo pharmacokinetic studies on the compound. Â© 2006 Elsevier B.V. All rights reserved.
Journal of pharmaceutical and biomedical analysis
Wang, Ting and Wang, Desuo, "High performance liquid chromatographic analysis of MS23 piperidine analog MSP001 in rat plasma" (2006). PCOM Scholarly Papers. 690.