Resveratrol potentiates genistein's antiadipogenic and proapoptotic effects in 3T3-L1 adipocytes

Document Type

Article

Publication Date

2007

Abstract

Genistein (G) and resveratrol (R) individually inhibit adipogenesis in 3T3-L1 adipocytes and induce apoptosis in cancer cells. We investigated whether the combination of G and R resulted in enhanced effects on adipogenesis, lipolysis, and apoptosis in 3T3-L1 cells. Preadipocytes and mature adipocytes were treated with G and R individually at 50 and 100 mmol/L (G100; R100) and in combination. Both in preadipocytes and mature adipocytes, G and R individually decreased cell viability dose-dependently, but G100 1 R100 further decreased viability by 59 ± 0.97% (P < 0.001) and 69.7 ± 1.2% (P < 0.001) after 48 h compared with G100 and R100, respectively. G100 + R100 induced apoptosis 242 ± 8.7% (P < 0.001) more than the control after 48 h, whereas G100 and R100 individually increased apoptosis only 46 ± 9.2 and 46 ± 7.9%, respectively. G and R did not modulate mitogen-activated protein kinase expression by themselves, but G100 + R100 increased Jun-N-terminal kinase phosphorylation by 38.8 ± 4.4% (P < 0.001) and decreased extracellular signal-regulating kinase phosphorylation by 48 ± 3.4% (P < 0.001). Individually, G and R at 25 μmol/L (G25; R25) decreased lipid accumulation by 30 ± 1.7% and 20.07 ± 4.27%, respectively (P < 0.001). However, G25 + R25 decreased lipid accumulation by 77.9 ± 3.4% (P < 0.001). Lipolysis assay revealed that neither G25 nor R25 induced lipolysis, whereas G25 + R25 significantly increased lipolysis by 25.5 ± 4.6%. The adipocyte-specific proteins PPARγ and CCAAT/enhancer binding protein-alpha were downregulated after treatment with G + R, but no effect was observed with individual compounds. These results indicate that G and R in combination produce enhanced effects on inhibiting adipogenesis, inducing apoptosis, and promoting lipolysis in 3T3-L1 adipocytes. Thus, the combination of G and R is more potent in exerting antiobesity effects than the individual compounds. © 2007 American Society for Nutrition.

Publication Title

Journal of Nutrition

Volume

137

Issue

12

First Page

2668

Last Page

2673

Comments

This article was published in Journal of Nutrition, Volume 137, Issue 12, Pages 2668-2673.

The published version is available at http://jn.nutrition.org/content/137/12/2668.abstract .

Copyright © 2007 American Society for Nutrition.

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