The Role of Tetrahydrobiopterin and Dihydrobiopterin in Ischemia/Reperfusion Injury When Given at Reperfusion

Authors

Qian Chen, Philadelphia College of Osteopathic MedicineFollow
Elizabeth Eun Jung Kim, Philadelphia College of Osteopathic MedicineFollow
Katrina Elio, Philadelphia College of Osteopathic MedicineFollow
Christopher Zambrano, Philadelphia College of Osteopathic MedicineFollow
Samuel Krass, Philadelphia College of Osteopathic MedicineFollow
Jane Chun-Wen Teng, Philadelphia College of Osteopathic MedicineFollow
Helen Kay, Philadelphia College of Osteopathic MedicineFollow
Kerry-Anne Perkins, Philadelphia College of Osteopathic MedicineFollow
Sailesh Pershad, Philadelphia College of Osteopathic MedicineFollow
Sloane McGraw, Philadelphia College of Osteopathic MedicineFollow
Jeffrey Emrich, Philadelphia College of Osteopathic MedicineFollow
Jovan S. Adams, Philadelphia College of Osteopathic MedicineFollow
Lindon H. Young, Philadelphia College of Osteopathic MedicineFollow

Document Type

Article

Publication Date

1-1-2010

Abstract

Reduced nitric oxide (NO) bioavailability and increased oxidative stress are major factors mediating ischemia/reperfusion (I/R) injury. Tetrahydrobiopterin (BH(4)) is an essential cofactor of endothelial NO synthase (eNOS) to produce NO, whereas dihydrobiopterin (BH(2)) can shift the eNOS product profile from NO to superoxide, which is further converted to hydrogen peroxide (H(2)O(2)) and cause I/R injury. The effects of BH(4) and BH(2) on oxidative stress and postreperfused cardiac functions were examined in ex vivo myocardial and in vivo femoral I (20 min)/R (45 min) models. In femoral I/R, BH(4) increased NO and decreased H(2)O(2) releases relative to saline control, and these effects correlated with improved postreperfused cardiac function. By contrast, BH(2) decreased NO release relative to the saline control, but increased H(2)O(2) release similar to the saline control, and these effects correlated with compromised postreperfused cardiac function. In conclusion, these results suggest that promoting eNOS coupling to produce NO and decrease H(2)O(2) may be a key mechanism to restore postreperfused organ function during early reperfusion.

Publication Title

Advances in Pharmacological Sciences

Volume

2010

Issue

Article ID 963914

First Page

963914

Last Page

963914

PubMed ID

21188246

Comments

This article was published in Advances in Pharmacological Sciences, 2010, Article ID 963914, 2010, 11 pages.

The published version is available at http://dx.doi.org/10.1155/2010/963914

Copyright © 2010 Qian Chen et al. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Recommended Citation

Chen, Qian; Kim, Elizabeth Eun Jung; Elio, Katrina; Zambrano, Christopher; Krass, Samuel; Teng, Jane Chun-Wen; Kay, Helen; Perkins, Kerry-Anne; Pershad, Sailesh; McGraw, Sloane; Emrich, Jeffrey; Adams, Jovan S.; and Young, Lindon H., "The Role of Tetrahydrobiopterin and Dihydrobiopterin in Ischemia/Reperfusion Injury When Given at Reperfusion" (2010). PCOM Scholarly Works. 51.
https://digitalcommons.pcom.edu/scholarly_papers/51