Some Like It Hot -Structural Changes in Extremophile Rubredoxin at 120 °C.

Authors

Tzanko Doukov
Igor Leontyev
Francis E. Jenney, Philadelphia College of Osteopathic MedicineFollow
Dominic George
Stephen P Cramer

Document Type

Article

Publication Date

11-24-2025

Abstract

How does the structure of a protein change as the temperature is raised from cryogenic conditions at 100 K to 393 K? Understanding the structure and dynamics of proteins under environmental extremes is relevant for human health, biotechnological applications, and our search for life elsewhere in the universe. Here we reveal the high temperature crystal structure of a hyperthermophilic (Pyrococcus furiosus) rubredoxin at 393 K (120 °C), together with multiple complementary structures down to 100 K. The results are compared with molecular dynamics calculations. Significant changes in H-bonding are observed. Discussions about high-temperature protein structure and stability need to recognize that low temperature structures may not represent the high temperature case.

Publication Title

Angewandte Chemie (International ed. in English)

PubMed ID

41287388

Comments

This article was published in Angewandte Chemie International Edition.

The published version is available at https://doi.org/10.1002/anie.202520302.

Copyright © 2025 The Author(s). CC BY 4.0.

Recommended Citation

Doukov, Tzanko; Leontyev, Igor; Jenney, Francis E.; George, Dominic; and Cramer, Stephen P, "Some Like It Hot -Structural Changes in Extremophile Rubredoxin at 120 °C." (2025). PCOM Scholarly Works. 2343.
https://digitalcommons.pcom.edu/scholarly_papers/2343 DOI: https://doi.org/10.1002/anie.202520302