Transdermal Delivery of Proteins Using a Combination of Iontophoresis and Microporation

Document Type

Article

Publication Date

5-1-2014

Abstract

Aim: This study aimed to investigate transdermal delivery of proteins using combination of microporation and iontophoresis (ITP). Materials & methods & results: Delivery of model protein, Alexa Fluor 555 bovine serum albumin conjugate (AF-BSA) using ITP alone, microneedle (MN) alone, and ITP plus MN combination was assessed using confocal microscopy. Compared to MN alone, combination of MN plus ITP significantly increased skin's penetration depth of AF-BSA (300 vs 110 μm) and achieved lateral distribution of the model protein. Average fluorescence intensity quantified around each microchannel was 23.7-fold (8.2-fold, in vivo) higher for combination treatment compared with MN alone, in vitro. After 1 h in vitro permeation study, the unlabeled BSA amount delivered across skin was found to be 0, 1.4, 0.63 and 14 μg by passive, MN alone, ITP alone and ITP plus MN combination delivery, respectively.

Publication Title

Therapeutic Delivery

Volume

5

Issue

5

First Page

525

Last Page

536

PubMed ID

24998272

Comments

This article was published in Therapeutic Delivery, Volume 5, Number 5, May 2014, Pages 398-407.

The published version is available at http://dx.doi.org/10.4155/tde.14.19

Copyright © 2014 Future Science

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