Preparation of Chick Striated Muscle Cultures

Authors

Camille DiLullo
Jacquelyn Gerhart, Philadelphia College of Osteopathic MedicineFollow
Mindy George-Weinstein, Philadelphia College of Osteopathic MedicineFollow

Document Type

Article

Publication Date

1-1-2000

Abstract

The maturation of striated muscle in primary cultures closely parallels the formation of striated muscle in vivo. Primary cultures thus serve as model systems for the study and manipulation of various aspects of muscle development including the regulation of gene expression, myofibril assembly, myocyte fusion, and myotube contraction. The following protocols provide instructions for the preparation of skeletal muscle cultures from either day 10 embryonic pectoralis muscle or day 2 somites and segmental plate mesoderm. Both methods will yield well-striated, multinucleated, contracting myotubes (Fig. 1) (1–4). Also included is a protocol for the preparation of cardiac cultures from day 7 embryonic heart. This method will yield well-striated, contracting cardiomyocytes (Fig. 1) (5,6).

Publication Title

Methods in Molecular Biology

Volume

137

First Page

337

Last Page

349

PubMed ID

10948549

Comments

This article was published in Methods in Molecular Biology, Volume 137, Pages 337-349.

The published version is available at http://dx.doi.org/1-59259-066-7-337.

Copyright © 2000.

Recommended Citation

DiLullo, Camille; Gerhart, Jacquelyn; and George-Weinstein, Mindy, "Preparation of Chick Striated Muscle Cultures" (2000). PCOM Scholarly Works. 1781.
https://digitalcommons.pcom.edu/scholarly_papers/1781