Document Type
Article
Publication Date
1-1-2017
Abstract
PURPOSE: To identify Myo/Nog cells in the adult retina and test their role in protecting retinal photoreceptors from light damage.
METHODS: Light damage was induced by exposing albino rats raised in dim cyclic light to 1000 lux light for 24 hours. In one group of rats, Myo/Nog cells were purified from rat brain tissue by magnetic cell sorting following binding of the G8 monoclonal antibody (mAb). These cells were injected into the vitreous humour of the eye within 2 hours following bright light exposure. Retinal function was assessed using full-field, flash electroretinogram (ERG) before and after treatment. The numbers of Myo/Nog cells, apoptotic photoreceptors, and the expression of glial fibrillary acidic protein (GFAP) in Muller cells were assessed by immunohistochemistry.
RESULTS: Myo/Nog cells were present in the undamaged retina in low numbers. Light induced damage increased their numbers, particularly in the choroid, ganglion cell layer and outer plexiform layer. Intravitreal injection of G8-positive (G8+) cells harvested from brain mitigated all the effects of light damage examined, i.e. loss of retinal function (ERG), death of photoreceptors and the stress-induced expression of GFAP in Muller cells. Some of the transplanted G8+ cells were integrated into the retina from the vitreous.
CONCLUSIONS: Myo/Nog cells are a subpopulation of cells that are present in the adult retina. They increase in number in response to light induced stress. Intravitreal injection of Myo/Nog cells was protective to the retina, in part, by reducing retinal stress as measured by the Muller cell response. These results suggest that Myo/Nog cells, or the factors they produce, are neuroprotective and may be therapeutic in neurodegenerative retinal diseases.
Publication Title
PLoS One
Volume
12
Issue
1
PubMed ID
28099524
Recommended Citation
Brandli, Alice; Gerhart, Jacquelyn; Sutera, Christopher K; Purushothuman, Sivaraman; George-Weinstein, Mindy; Stone, Jonathan; and Bravo Nuevo, Arturo, "Role of Myo/Nog Cells in Neuroprotection: Evidence from the Light Damaged Retina." (2017). PCOM Scholarly Works. 1767.
https://digitalcommons.pcom.edu/scholarly_papers/1767
Comments
This article was published in PloS one, Volume 12, Issue 1.
The published version is available at http://dx.doi.org/10.1371/journal.pone.0169744.
Copyright © 2017. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.