Document Type

Article

Publication Date

2010

Abstract

We investigated an alternative pathway for emergence of the mesenchymal cells involved in epithelial sheet wound healing and a source of myofibroblasts that cause fibrosis. Using a mock cataract surgery model, we discovered a unique subpopulation of polyploid mesenchymal progenitors nestled in small niches among lens epithelial cells that expressed the surface antigen G8 and mRNA for the myogenic transcription factor MyoD. These cells rapidly responded to wounding of the lens epithelium with population expansion, acquisition of a mesenchymal phenotype, and migration to the wound edges where they regulate the wound response of the epithelium. These mesenchymal cells also were a principal source of myofibroblasts that emerged following lens injury and were responsible for fibrotic disease of the lens that occurs following cataract surgery. These studies provide insight into the mechanisms of wound-healing and fibrosis.

Publication Title

PNAS

Volume

107

Issue

31

Comments

This article was published in Proceedings of the National Academy of Sciences, Volume 107, Issue 31, Pages 13730 - 13735.

The published version is available at http://dx.doi.org/10.1073/pnas.0910382107

Copyright © 2010 Walker et al.

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