Inhibition of rat liver arginase by an intermediate in NO biosynthesis, N(G)-hydroxy-L-arginine: Implications for the regulation of nitric oxide biosynthesis by arginase

Authors

Farzaneh Daghigh, Philadelphia College of Osteopathic MedicineFollow
J. M. Fukoto
D. E. Ash

Document Type

Article

Publication Date

1994

Abstract

N(G)-hydroxy-L-arginine, an intermediate in the biosynthesis of nitric oxide (NO), has been found to be a uniquely potent competitive inhibitor of rat liver arginase. Among previously reported inhibitors of arginase and the eight arginine analogs tested herein, only N(G)-hydroxy-L-arginine was found to be strongly inhibitory. Significantly, the K(i) (42 μM) for inhibition of rat liver arginase by N(G)-hydroxy-L-arginine was found to be 20-40-fold lower than the K(M) (1-1.7 mM) for its natural substrate, L-arginine. Since N(G)-hydroxy-L-arginine is the only known intermediate in the biosynthesis of NO from L-arginine, this finding may have significant implications for the regulation of NO levels in tissues or cells, such as liver or macrophages, which synthesize both NO and contain arginase.

Publication Title

Biochemical and biophysical research communications

Volume

202

Issue

1

First Page

174

Last Page

180

Comments

This article was published in Biochemical and biophysical research communications, Volume 202, Issue 1, Pages 174-180.

Copyright © 1994 Elsevier.

Recommended Citation

Daghigh, Farzaneh; Fukoto, J. M.; and Ash, D. E., "Inhibition of rat liver arginase by an intermediate in NO biosynthesis, N(G)-hydroxy-L-arginine: Implications for the regulation of nitric oxide biosynthesis by arginase" (1994). PCOM Scholarly Works. 1035.
https://digitalcommons.pcom.edu/scholarly_papers/1035