Location
Suwanee, GA
Start Date
11-5-2023 1:00 PM
End Date
11-5-2023 4:00 PM
Description
Introduction: While the effects of ethanol on humans are well known, its influence on SMN2 mice, specifically on motor movement and coordination, have not been established.
Objectives: The primary objectives are to determine how varying levels of ethanol consumption alters: 1) the motor ability of SMN2 mice, 2) the time window of coordination impairment, and 3) how ethanol influences free-field behavior.
Methods: Motor impairment in SMN2 mice were measured with a RotoRod test (to test for coordination), and a free-field test (to monitor free movement and behavior). For each test, groups of animals were orally gavaged different concentrations of ethanol prior to testing. Blood alcohol concentrations (BAC) were measured for each concentration given to provide a baseline measure for the amount of alcohol consumed. In the RotoRod test, the number of falls from the rod was recorded at varying intervals after gavage for each concentration given. For the free-field behavioral testing, animals were monitored and recorded for movement ability in a plexiglass box.
Results:Results showed statistically significant motor impairment for mice gavaged 75% ethanol. This treatment level produced a BAC level of 0.14 g/dl at 30-45 minutes post gavage. Animals gavaged 95% ethanol or higher consistently fell off of the rod within 5-10 seconds of placement (p
Conclusions: These experiments show that: 1) SMN2 mice require a significantly higher blood alcohol concentration to influence motor ability than the human population, and 2) the effects of ethanol are quickly eliminated from their systems. This may be due to a very high metabolism in these animals.
Embargo Period
7-26-2024
Included in
Determining the Concentration of Ethanol Needed for Motor Impairment in SMN2 Mice
Suwanee, GA
Introduction: While the effects of ethanol on humans are well known, its influence on SMN2 mice, specifically on motor movement and coordination, have not been established.
Objectives: The primary objectives are to determine how varying levels of ethanol consumption alters: 1) the motor ability of SMN2 mice, 2) the time window of coordination impairment, and 3) how ethanol influences free-field behavior.
Methods: Motor impairment in SMN2 mice were measured with a RotoRod test (to test for coordination), and a free-field test (to monitor free movement and behavior). For each test, groups of animals were orally gavaged different concentrations of ethanol prior to testing. Blood alcohol concentrations (BAC) were measured for each concentration given to provide a baseline measure for the amount of alcohol consumed. In the RotoRod test, the number of falls from the rod was recorded at varying intervals after gavage for each concentration given. For the free-field behavioral testing, animals were monitored and recorded for movement ability in a plexiglass box.
Results:Results showed statistically significant motor impairment for mice gavaged 75% ethanol. This treatment level produced a BAC level of 0.14 g/dl at 30-45 minutes post gavage. Animals gavaged 95% ethanol or higher consistently fell off of the rod within 5-10 seconds of placement (p
Conclusions: These experiments show that: 1) SMN2 mice require a significantly higher blood alcohol concentration to influence motor ability than the human population, and 2) the effects of ethanol are quickly eliminated from their systems. This may be due to a very high metabolism in these animals.