High performance liquid chromatographic analysis of MS23 piperidine analog MSP001 in rat plasma

Authors

Ting Wang
Desuo Wang, Philadelphia College of Osteopathic MedicineFollow

Document Type

Article

Publication Date

2006

Abstract

MSP001 is a newly synthesized piperidine analog of the lead antihypertensive compound MS23 that dually targets cAMP-specific type 4 phosphodiesterase and L-type calcium channels. We validated an analytical protocol for MSP001 in rat plasma using high performance liquid chromatographic method. A C18 column and a phosphate/acetonitrile buffer were used to perform the chromatographic separation. UV detection was carried out at 307 nm, a wavelength at which an absorption peak was detected for this group of compounds. The calibration curve for MSP001 was linear in the range from 25 to 10,000 ng/ml. The limit of quantification (LOQ) was 25 ng/ml. The results demonstrate that this method has high linearity (R = 0.99995), compound specificity, and acceptable precision/accuracy. The protocol is suitable for in vivo pharmacokinetic studies on the compound. © 2006 Elsevier B.V. All rights reserved.

Publication Title

Journal of pharmaceutical and biomedical analysis

Volume

42

Issue

5

First Page

607

Last Page

612

Comments

This article was published in Journal of pharmaceutical and biomedical analysis, Volume 42, Issue 5, Pages 607-612.

Copyright © 2006 Scopus.

Recommended Citation

Wang, Ting and Wang, Desuo, "High performance liquid chromatographic analysis of MS23 piperidine analog MSP001 in rat plasma" (2006). PCOM Scholarly Works. 690.
https://digitalcommons.pcom.edu/scholarly_papers/690