MyoD-Positive Epiblast Cells Regulate Skeletal Muscle Differentiation in the Embryo
MyoD mRNA is expressed in a subpopulation of cells within the embryonic epiblast. Most of these cells are incorporated into somites and synthesize Noggin. Ablation of MyoD-positive cells in the epiblast subsequently results in the herniation of organs through the ventral body wall, a decrease in the expression of Noggin, MyoD, Myf5, and myosin in the somites and limbs, and an increase in Pax-3-positive myogenic precursors. The addition of Noggin lateral to the somites compensates for the loss of MyoD-positive epiblast cells. Skeletal muscle stem cells that arise in the epiblast are utilized in the somites to promote muscle differentiation by serving as a source of Noggin.
The Journal of Cell Biology
Gerhart, Jacquelyn; Elder, Justin; Neely, Christine; Schure, Jared; Kvist, Tage; Knudsen, Karen; and George-Weinstein, Mindy, "MyoD-Positive Epiblast Cells Regulate Skeletal Muscle Differentiation in the Embryo" (2006). PCOM Scholarly Papers. 98.