Cardiopulmonary bypass increases coronary IL-8 in diabetic patients without evidence of reperfusion injury
Document Type
Article
Publication Date
1999
Abstract
Background. Endothelin-1 (ET-1) has been shown to be a potent agonist for monocyte production of the neutrophil chemotactic cytokine interleukin-8 (IL-8). We have shown that diabetic patients demonstrate elevated coronary ET-1 after coronary artery bypass grafting (CABG). We hypothesized that these same diabetic patients would manifest elevated coronary IL-8 and conjugated diene concentrations (an index of reperfusion injury). Methods. Sixteen patients [9 nondiabetics and 7 type II diabetics] underwent nonemergent CABG. The two groups did not differ significantly in preoperative ejection fraction, number of vessels bypassed, or cross-clamp time. Coronary sinus samples were obtained prior to cardioplegic arrest (baseline) and at 1 and 15 min after reperfusion periods A and B (A, reperfusion of native coronaries + LIMA; B, reperfusion of saphenous vein grafts in addition to native coronary system + LIMA). Plasma samples were analyzed for IL-8 (ELISA) and conjugated dienes (spectrophotometry). Results. Initially after reperfusion, IL-8 in both groups was significantly lower than precardioplegia values. In reperfusion B, only the diabetic group demonstrated a significant increase in IL-8 concentrations at 1 and 15 min compared to nondiabetics. Conjugated diene levels were significantly higher in diabetics at each time point than nondiabetics. Conclusions. This study demonstrates an early decrease in IL-8 in both groups, most likely related to depressed production secondary to hypothermia. The subsequent elevation in IL-8 only in the diabetic group was seen without concomitant conjugated diene elevation. While no evidence of reperfusion injury was demonstrated in this time frame, the elevation of IL-8 in diabetics after CABG may contribute to later infiltration and associated oxidative damage.
Publication Title
Journal of Surgical Research
Volume
84
First Page
46
Last Page
50
Recommended Citation
Nawas, S. I.; Doherty, J. C.; Vigneswaran, W. T.; Fogelson, B. G.; McMillen, M. A.; Ferguson, J. L.; Sharma, Avadhesh C.; and Law, W. R., "Cardiopulmonary bypass increases coronary IL-8 in diabetic patients without evidence of reperfusion injury" (1999). PCOM Scholarly Papers. 951.
https://digitalcommons.pcom.edu/scholarly_papers/951
Comments
This article was published in Journal of Surgical Research, Volume 84, Issue 1, Pages 46-50.
The published version is available at http://dx.doi.org/10.1006/jsre.1999.5602.Copyright © 1999.