Validation of HPLC analysis method of a novel antihypertensive agent MS23 in rat plasma
MS23 is a vasodilator with unique dual action pharmacological profile to inhibit type 4 PDE and antagonize L-type calcium channels. We validated an analytical protocol for MS23 in rat plasma using high performance liquid chromatography (HPLC). A C18 column and a phosphate/acetonitrite buffer were used for chromatographic separation. UV detection was performed at 307 nm. The calibration curve for MS23 was linear in the range from 50 to 10,000 ng/ml. The limit of quantification (LOQ) was 50 ng/ml. The results demonstrate that the method has linearity (R = 0.9989), specificity, and acceptable precision/accuracy. This method is simple, economic, and sufficient for in vivo pharmacokinetic studies on the compound. Â© 2005 Elsevier B.V. All rights reserved.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Wang, Ting; Fox, Laura M.; and Wang, Desuo, "Validation of HPLC analysis method of a novel antihypertensive agent MS23 in rat plasma" (2006). PCOM Scholarly Papers. 689.