Identification of protein-bound dinitrosyl iron complexes by nuclear resonance vibrational spectroscopy

Z. J. Tonzetich
H. Wang
D. Mitra
C. E. Tinberg
L H. Do
Francis E. Jenney Jr., Philadelphia College of Osteopathic Medicine
M. W. Adams
S. P. Cramer
S. J. Lippard

This article was published in Journal of the American Chemical Society, Volume 132, Issue 20, Pages 6914-6916.

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Copyright © 2010 ACS.


We have applied 57Fe nuclear resonance vibrational spectroscopy (NRVS) to identify protein-bound dinitrosyl iron complexes. Intense NRVS peaks due to vibrations of the N-Fe-N unit can be observed between 500 and 700 cm -1 and are diagnostic indicators of the type of iron dinitrosyl species present. NRVS spectra for four iron dinitrosyl model compounds are presented and used as benchmarks for the identification of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide. © 2010 American Chemical Society.