Structure of the hypothetical protein PF0899 from Pyrococcus furiosus at 1.85 Ã… resolution

L. Clancy Kelley
B. D. Dillard
W. Tempel
L. Chen
N. Shaw
D. Lee
M. Newton
F. J. Sugar
Francis E. Jenney Jr., Philadelphia College of Osteopathic Medicine
et al.

This article was published in Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Volume 63, Issue 7, Pages 549-552.

The published version is available at

Copyright © 2007 IUCr.


The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics ( The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 Å against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and Rfree of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein. © International Union of Crystallography 2007.