Structure of the Hypothetical Protein PF0899 from Pyrococcus furiosus at 1.85 A Resolution
The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications
Kelley, Laura-Lee Clancy; Dillard, Bret D.; Tempel, Wolfram; Chen, Lirong; Shaw, Neil; Lee, Doowon; Newton, M. Gary; Sugar, Frank J.; Jenney, Francis E.; Lee, Han Seung; Shah, Claudia; Poole, Farris L.; Adams, Michael W. W.; Richardson, Jane S.; Richardson, David C.; Liu, Zhi-Jie; Wang, Bi-Cheng; and Rose, John, "Structure of the Hypothetical Protein PF0899 from Pyrococcus furiosus at 1.85 A Resolution" (2007). PCOM Scholarly Papers. 200.