Location

Philadelphia, PA

Start Date

10-5-2021 12:00 AM

End Date

13-5-2021 12:00 AM

Description

Introduction: Melanoma is predicted to be the fifth most common cause of cancer mortality in the U.S. in 2021. Although melanoma affects both sexes, current literature shows it is more prevalent and more deadly in men. Several potential reasons for this difference have been proposed, but most literature describes a gap in male knowledge concerning skin cancer and unwillingness to go to the dermatologist as an explanation. That said, the androgen receptor has been proven to play a role in melanoma growth and metastasis, and may play a part in the difference in incidence and mortality rates between sexes. Resveratrol, a naturally occurring compound found in grapes and berries with poor bioavailability, has been shown to decrease melanoma cell growth in both in vitro and in vivo studies. Its effects on the expression of several genes involved in the androgen receptor pathway in non-melanoma cancer are well documented. Although several mechanisms have been proposed in which resveratrol effects melanoma cell growth, we suspect the androgen receptor pathway may be another target that warrants further investigation.

Objective: Our objective is to reiterate the potential for resveratrol as a treatment in melanoma and to propose that it changes melanoma behavior at least partially through its interaction in the androgen receptor pathway. We also will discuss pros and cons of the various ways resveratrol may be delivered as a treatment for melanoma.

Methods: To support our hypothesis we reviewed current literature and performed scratch wound assay, qPCR, and Western Blotting.

Results: Melanoma cells treated with resveratrol showed a decrease in the distance traveled during scratch wound assay. Additionally, androgen receptor pathway gene expression and protein level was altered in cells treated with resveratrol.

Conclusions: The androgen receptor pathway plays a role in melanoma cell growth and metastasis. Resveratrol is a potential therapeutic that decreases melanoma growth at least partially through its effects on this pathway. Further melanoma in vivo studies that explore the various methods of treatment with resveratrol are needed to help the transition from bench to bedside.

Embargo Period

6-3-2021

COinS
 
May 10th, 12:00 AM May 13th, 12:00 AM

Resveratrol's Effect on the Androgen Receptor Pathway as a Target for Decreasing Growth and Invasion of Melanoma and Methods of Application

Philadelphia, PA

Introduction: Melanoma is predicted to be the fifth most common cause of cancer mortality in the U.S. in 2021. Although melanoma affects both sexes, current literature shows it is more prevalent and more deadly in men. Several potential reasons for this difference have been proposed, but most literature describes a gap in male knowledge concerning skin cancer and unwillingness to go to the dermatologist as an explanation. That said, the androgen receptor has been proven to play a role in melanoma growth and metastasis, and may play a part in the difference in incidence and mortality rates between sexes. Resveratrol, a naturally occurring compound found in grapes and berries with poor bioavailability, has been shown to decrease melanoma cell growth in both in vitro and in vivo studies. Its effects on the expression of several genes involved in the androgen receptor pathway in non-melanoma cancer are well documented. Although several mechanisms have been proposed in which resveratrol effects melanoma cell growth, we suspect the androgen receptor pathway may be another target that warrants further investigation.

Objective: Our objective is to reiterate the potential for resveratrol as a treatment in melanoma and to propose that it changes melanoma behavior at least partially through its interaction in the androgen receptor pathway. We also will discuss pros and cons of the various ways resveratrol may be delivered as a treatment for melanoma.

Methods: To support our hypothesis we reviewed current literature and performed scratch wound assay, qPCR, and Western Blotting.

Results: Melanoma cells treated with resveratrol showed a decrease in the distance traveled during scratch wound assay. Additionally, androgen receptor pathway gene expression and protein level was altered in cells treated with resveratrol.

Conclusions: The androgen receptor pathway plays a role in melanoma cell growth and metastasis. Resveratrol is a potential therapeutic that decreases melanoma growth at least partially through its effects on this pathway. Further melanoma in vivo studies that explore the various methods of treatment with resveratrol are needed to help the transition from bench to bedside.