Title

Genistein inhibits differentiation of primary human adipocytes

Document Type

Article

Publication Date

2009

Abstract

Genistein, a major soy isoflavone, has been reported to exhibit antiadipogenic and proapoptotic potential in vivo and in vitro. It is also a phytoestrogen which has high affinity to estrogen receptor β. In this study, we determined the effect of genistein on adipogenesis and estrogen receptor (ER) α and β expression during differentiation in primary human preadipocytes. Genistein inhibited lipid accumulation in a dose-dependent manner at concentrations of 6.25 μM and higher, with 50 μM genistein inhibiting lipid accumulation almost completely. Low concentrations of genistein (3.25 μM) increased cell viability and higher concentrations (25 and 50 μM) decreased it by 16.48±1.35% (P<.0001) and 50.68±1.34% (P<.0001). Oil Red O staining was used to confirm the effects on lipid accumulation. The inhibition of lipid accumulation was associated with inhibition of glycerol-3-phosphate dehydrogenase activity and down-regulation of expression of adipocyte-specific genes, including peroxisome proliferator-activated receptor γ, CCAAT/enhancer binding protein α, glycerol-3-phosphate dehydrogenase, adipocyte fatty acid binding protein, fatty acid synthase, sterol regulatory element-binding protein 1, perilipin, leptin, lipoprotein lipase and hormone-sensitive lipase. These effects of genistein during the differentiation period were associated with down-regulation of ERα and ERβ expression. This study adds to the elucidation of the molecular pathways involved in the inhibition of adipogenesis by phytoestrogens. © 2009 Elsevier Inc. All rights reserved.

Publication Title

Journal of Nutritional Biochemistry

Volume

20

Issue

2

First Page

140

Last Page

148

Comments

This article was published in Journal of Nutritional Biochemistry, Volume 20, Issue 2, Pages 140-148.

The published version is available at http://dx.doi.org/10.1016/j.jnutbio.2008.01.006.

Copyright © 2009 Elsevier.

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