Ajoene exerts potent effects in 3t3-l1 adipocytes by inhibiting adipogenesis and inducing apoptosis i

Document Type

Article

Publication Date

2009

Abstract

This paper describes effects of several sulfur-containing compounds from garlic on the cell viability, apoptosis and adipogenesis in 3T3-L1 adipocytes. In both preadipocytes and mature adipocytes, 100 and 200 |iM ajoene significantly decreased cell viability and increased apoptosis. The effect on apoptosis was further confirmed with Hoechst staining. In contrast, diallyl sulfide, diallyl disulfide, diallyl trisulfide, deoxyalliin, and allyl methyl sulfide had no significant effect on cell viability or apoptosis in either preadipocytes or mature adipocytes. In maturing preadipocytes ajoene significantly decreased lipid accumulation in a dose-dependent manner and these results were further confirmed by a decrease in lipid droplet number and lipid content through Oil Red O staining. There was no significant change in lipid accumulation in maturing preadipocytes treated with other garlic derivatives. Thus, despite the same source of origin, garlic, ajoene was the only one with potent effects on cell viability, apoptosis and adipogenesis in 3T3-L1 adipocytes. Copyright © 2008 John Wiley & Sons, Ltd.

Publication Title

Phytotherapy Research

Volume

23

Issue

4

First Page

513

Last Page

518

Comments

This article was published in Phytotherapy Research, Volume 23, Issue 4, Pages 513-518.

The published version is available at http://dx.doi.org/10.1002/ptr.2663.

Copyright © 2009 Wiley.

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