Strain-specific differences in formation of apoptotic DNA ladders in MCF-7 breast cancer cells
We tested the ability of seven MCF-7 strains to undergo DNA fragmentation, as measured by DNA laddering, following doxorubicin-induced apoptosis. Four strains were found to undergo DNA laddering while three were not. All strains were inhibited by doxorubicin, although sensitivity differed. Finally, we show by detection of sub-G1 DNA that doxorubicin induced the same fold increase in apoptosis in MCF-7-ATCC, which did not ladder, and MCF-7-MG, which did ladder. Therefore, detection of DNA ladders is not an accurate indicator of apoptosis in MCF-7 cells as fragmentation of DNA appears to vary between strains.
Gooch, Jennifer L. and Yee, D., "Strain-specific differences in formation of apoptotic DNA ladders in MCF-7 breast cancer cells" (1999). PCOM Scholarly Papers. 492.
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