Title

Murine coronavirus spike protein determines the ability of the virus to replicate in the liver and cause hepatitis

Document Type

Article

Publication Date

2001

Abstract

Recombinant mouse hepatitis viruses (MHV) differing only in the spike gene, containing A59, MHV-4, and MHV-2 spike genes in the background of the A59 genome, were compared for their ability to replicate in the liver and induce hepatitis in weanling C57BL/6 mice infected with 500 PFU of each virus by intrahepatic injection. Penn98-1, expressing the MHV-2 spike gene, replicated to high titer in the liver, similar to MHV-2, and induced severe hepatitis with extensive hepatocellular necrosis. SA59R13, expressing the A59 spike gene, replicated to a somewhat lower titer and induced moderate to severe hepatitis with zonal necrosis, similar to MHV-A59. S4R21, expressing the MHV-4 spike gene, replicated to a minimal extent and induced few if any pathological changes, similar to MHV-4. Thus, the extent of replication and the degree of hepatitis in the liver induced by these recombinant viruses were determined largely by the spike protein.

Publication Title

Journal of virology

Volume

75

Issue

5

First Page

2452

Last Page

2457

Comments

This article was published in Journal of virology, Volume 75, Issue 5, Pages 2452-2457.

The published version is available at http://dx.doi.org/10.1128/JVI.75.5.2452-2457.2001.

Copyright © 2001 ASM.

This document is currently not available here.

COinS