Strain-specific differences in formation of apoptotic DNA ladders in MCF-7 breast cancer cells

Document Type

Article

Publication Date

1999

Abstract

We tested the ability of seven MCF-7 strains to undergo DNA fragmentation, as measured by DNA laddering, following doxorubicin-induced apoptosis. Four strains were found to undergo DNA laddering while three were not. All strains were inhibited by doxorubicin, although sensitivity differed. Finally, we show by detection of sub-G1 DNA that doxorubicin induced the same fold increase in apoptosis in MCF-7-ATCC, which did not ladder, and MCF-7-MG, which did ladder. Therefore, detection of DNA ladders is not an accurate indicator of apoptosis in MCF-7 cells as fragmentation of DNA appears to vary between strains.

Publication Title

Cancer letters

Volume

144

Issue

1

First Page

31

Last Page

37

Comments

This article was published in Cancer letters, Volume 144, Issue 1, Pages 31-37.

The published version is available at http://dx.doi.org/10.1016/S0304-3835(99)00208-6.

Copyright © 1999 Elsevier.

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