Title

High performance liquid chromatographic analysis of MS23 piperidine analog MSP001 in rat plasma

Document Type

Article

Publication Date

2006

Abstract

MSP001 is a newly synthesized piperidine analog of the lead antihypertensive compound MS23 that dually targets cAMP-specific type 4 phosphodiesterase and L-type calcium channels. We validated an analytical protocol for MSP001 in rat plasma using high performance liquid chromatographic method. A C18 column and a phosphate/acetonitrile buffer were used to perform the chromatographic separation. UV detection was carried out at 307 nm, a wavelength at which an absorption peak was detected for this group of compounds. The calibration curve for MSP001 was linear in the range from 25 to 10,000 ng/ml. The limit of quantification (LOQ) was 25 ng/ml. The results demonstrate that this method has high linearity (R = 0.99995), compound specificity, and acceptable precision/accuracy. The protocol is suitable for in vivo pharmacokinetic studies on the compound. © 2006 Elsevier B.V. All rights reserved.

Publication Title

Journal of pharmaceutical and biomedical analysis

Volume

42

Issue

5

First Page

607

Last Page

612

Comments

This article was published in Journal of pharmaceutical and biomedical analysis, Volume 42, Issue 5, Pages 607-612.

The published version is available at http://dx.doi.org/10.1016/j.jpba.2006.05.010.

Copyright © 2006 Scopus.

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