Identification of Protein-Bound Dinitrosyl Iron Complexes by Nuclear Resonance Vibrational Spectroscopy

Authors

Zachary J. Tonzetich
Hongxin Wang
Devrani Mitra
Christine E. Tinberg
Loi H. Do
Francis E. Jenney, Philadelphia College of Osteopathic MedicineFollow
Michael W. W. AdamsFollow
Stephen P. Cramer
Stephen J. Lippard

Document Type

Article

Publication Date

5-26-2010

Abstract

We have applied (57)Fe nuclear resonance vibrational spectroscopy (NRVS) to identify protein-bound dinitrosyl iron complexes. Intense NRVS peaks due to vibrations of the N-Fe-N unit can be observed between 500 and 700 cm(-1) and are diagnostic indicators of the type of iron dinitrosyl species present. NRVS spectra for four iron dinitrosyl model compounds are presented and used as benchmarks for the identification of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide.

Publication Title

Journal of the American Chemical Society

Volume

132

Issue

20

First Page

6914

Last Page

6916

PubMed ID

20429508

Comments

This article was published in Journal of the American Chemical Society, Volume 132, Issue 20, May 26, 2010, Pages 6914-6.

The published version is available at http://dx.doi.org/10.1021/ja101002f

Copyright © 2010 American Chemical Society

Recommended Citation

Tonzetich, Zachary J.; Wang, Hongxin; Mitra, Devrani; Tinberg, Christine E.; Do, Loi H.; Jenney, Francis E.; Adams, Michael W. W.; Cramer, Stephen P.; and Lippard, Stephen J., "Identification of Protein-Bound Dinitrosyl Iron Complexes by Nuclear Resonance Vibrational Spectroscopy" (2010). PCOM Scholarly Papers. 67.
https://digitalcommons.pcom.edu/scholarly_papers/67