A neutron crystallographic analysis of a rubredoxin mutant
Document Type
Article
Publication Date
2002
Abstract
In order to study the unusual thermostability of rubredoxin from Pyrococcus furiosus (RubPf), the structure of a 'triple mutant' of this rubredoxin, which is less thermostable than the wild type, was solved at 1.5-Å resolution by neutron-diffraction analysis using the BIX-3 diffractometer at the JRR-3M reactor of JAERI. The positions of the non-hydrogen atoms are almost the same as the native rubredoxin; however, for Trp3 → Tyr3, large structural changes were found, so that their hydrogen-bonding schemes are significantly different. Some positions of the hydrogen atoms and molecules of hydration are shifted in certain regions, suggesting that such differences may contribute to the differences in thermostability between this 'triple-mutant' rubredoxin and wild-type rubredoxin.
Publication Title
Applied Physics A: Materials Science and Processing
Volume
74
Issue
SUPPL.II
First Page
S1280
Last Page
S1282
Recommended Citation
Chatake, T.; Kurihara, K.; Tanaka, I.; Adams, M. W.; Jenney, Francis E. Jr.; Tsyba, I.; Bau, R.; and Niimura, N., "A neutron crystallographic analysis of a rubredoxin mutant" (2002). PCOM Scholarly Papers. 569.
https://digitalcommons.pcom.edu/scholarly_papers/569
Comments
This article was published in Applied Physics A: Materials Science and Processing, Volume 74, Issue SUPPL.II, Pages S1280-S1282.
The published version is available at http://dx.doi.org/10.1007/s003390201696.Copyright © 2002 Springer.