Title

Rhamnolipid from Pseudomonas aeruginosa inactivates mammalian tracheal ciliary axonemes.

Document Type

Article

Publication Date

1986

Abstract

Isolated ciliary axonemes from pig trachea were exposed to increasing concentrations of purified Pseudomonas aeruginosa rhamnolipid. This is a defined ciliary system allowing observation of direct impairment of functional axonemes. Axonemal motility and ATPase activity were decreased in proportion to rhamnolipid concentrations. ATPase-associated proteins observed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and dynein arms seen in ultra-structural cross sections progressively disappeared from axonemes with exposure to rhamnolipid. These four independent measures establish that the rhamnolipid removes the ATPase-containing outer dynein arms from the ciliary axoneme, thereby rendering the axoneme immotile.

Publication Title

Cell motility and the cytoskeleton

Volume

6

Issue

5

First Page

502

Last Page

509

Comments

This article was published in Cell motility and the cytoskeleton, Volume 6, Issue 5, Pages 502-509.

The published version is available at http://dx.doi.org/10.1002/cm.970060509 .

Copyright © 1986 Wiley.

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