Fluorescence Recovery after Photobleaching (FRAP) with a Focus on F-Actin

Authors

Lori R. Hardy, Philadelphia College of Osteopathic MedicineFollow

Document Type

Book

Publication Date

1-1-2012

Abstract

In this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein-protein interactions in situ is described. The protocols presented use confocal microscopy to examine the mobility of a fluorescent protein by measuring the recovery of the protein in a bleached area. The data gained in FRAP studies is qualitative and yields insight into relative binding affinity, binding characteristics, and the effect of treatments or mutations on protein mobility.

Publication Title

Current Protocols in Neuroscience

Volume

Chapter 2

First Page

2

Last Page

2

PubMed ID

23093350

Comments

This article was published in Current Protocols in Neuroscience, Chapter 2, Unit 2.17, 2012.

The published version is available at http://dx.doi.org/10.1002/0471142301.ns0217s61

Copyright © 2012 by John Wiley & Sons, Inc.

Recommended Citation

Hardy, Lori R., "Fluorescence Recovery after Photobleaching (FRAP) with a Focus on F-Actin" (2012). PCOM Scholarly Papers. 196.
https://digitalcommons.pcom.edu/scholarly_papers/196