Document Type
Article
Publication Date
1-1-2017
Abstract
1[2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oyl]imidazole (CDDO-Im), a synthetic derivative of oleanolic acid that exhibits antioxidant and anti-inflammatory activity in several animal and in vitro models, has been shown to be beneficial if given after injury. Although induction of heme oxygenase 1 appears to be a major effector of cytoprotection, the mechanism by which the overall effect is mediated is largely unknown. This study evaluated temporal gene expression profiles to better characterize the early transcriptional events and their relationship to the dynamics of the cytoprotective response in human umbilical vein endothelial cells (HUVEC) to CDDO-Im. Time-course gene expression profiling was performed on HUVEC treated with CDDO-Im for 0.5, 1, 3, 6, and 24 hours. More than 10 000 genes were statistically altered in their expression in at least 1 time point across the time course. Large alterations in immediate-early gene expression were readily detectable within 0.5 hour after administration of CDDO-Im.
Publication Title
Gene Regulation and Systems Biology
Volume
11
PubMed ID
28469413
Recommended Citation
Bynum, James A; Wang, Xinyu; Stavchansky, Salomon A; and Bowman, Phillip D, "Time Course Expression Analysis of 1[2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oyl]imidazole Induction of Cytoprotection in Human Endothelial Cells." (2017). PCOM Scholarly Papers. 1812.
https://digitalcommons.pcom.edu/scholarly_papers/1812
Comments
This article was published in Gene Regulation and Systems Biology, Volume 11.
The published version is available at http://dx.doi.org/10.1177/1177625017701106.
Copyright © 2017. (CC BY-NC 3.0)