Title

Epiblast cells that express MyoD recruit pluripotent cells to the skeletal muscle lineage

Document Type

Article

Publication Date

2004

Abstract

Embryonic stem cells are derived from the epiblast. A subpopulation of epiblast cells expresses MyoD mRNA and the G8 antigen in vivo. G8 positive (G8pos) and G8 negative (G8neg) populations were isolated by magnetic cell sorting. Nearly all G8pos cells switched from E- to N-cadherin and differentiated into skeletal muscle in culture. G8neg cells were impaired in their ability to switch cadherins and few formed skeletal muscle. Medium conditioned by G8pos cells stimulated skeletal myogenesis and N-cadherin synthesis in G8neg cultures. The effect of conditioned medium from G8pos cultures was inhibited by bone morphogenetic protein (BMP) 4. Treatment of G8neg cells with a soluble form of the BMP receptor-1A or Noggin promoted N-cadherin synthesis and skeletal myogenesis. These results demonstrate that MyoD-positive epiblast cells recruit pluripotent cells to the skeletal muscle lineage. The mechanism of recruitment involves blocking the BMP signaling pathway.

Publication Title

Journal of Cell Biology

Volume

164

Issue

5

First Page

739

Last Page

746

Comments

This article was published in Journal of Cell Biology, Volume 164, Issue 5, Pages 739-746.

The published version is available at http://dx.doi.org/10.1083/jcb.200309152.

Copyright © 2004 Rockefeller University Press.

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