Title

Loss of adenosine-induced negative inotropic effect in hyperexcited rabbit hearts: Relationship to PKC

Document Type

Article

Publication Date

1995

Abstract

Adenosine produced a negative inotropic effect in hearts isolated from calm rabbits but not from those exhibiting alarm behavior during handling. This study was conducted to determine whether protein kinase C (PKC) activation is responsible for the loss of adenosine-induced negative inotropism in the hearts of hyperexcited rabbits. Adenosine (10 μM) decreased myocardial contractility (dP/dt(max)) in the hearts of calm, but not hyperexcited, rabbits but decreased heart rate (HR) and coronary perfusion pressure (PP) in the hearts of both calm and hyperexcited animals. During infusion of calphostin C (200 nM), a PKC inhibitor, adenosine also decreased dP/dt(max) in the hearts of hyperexcited rabbits. Calphostin C did not alter the actions of adenosine in the hearts of calm rabbits. Agents that stimulate PKC directly [phorbol 12,13-dibutyrate (PDBu), 1 nM] or indirectly [norepinephrine (NE), 3 nM; angiotensin II (ANG II), 5 nM] abolished the adenosine-induced decrease in dP/dt(max) but not HR or PP in the hearts of calm rabbits. During calphostin C, infusion of PDBu, NE, and ANG II failed to prevent the adenosine-induced decrease in dP/dt(max). These data suggest that the lack of a negative inotropic effect of adenosine in hyperexcited rabbits is due to an increase in PKC activity.

Publication Title

American Journal of Physiology - Heart and Circulatory Physiology

Volume

268

Issue

3 37-3

First Page

H1037

Last Page

H1044

Comments

This article was published in American Journal of Physiology - Heart and Circulatory Physiology, Volume 268, Issue 3 37-3, Pages H1037-H1044.

The published version is available at http://ajpheart.physiology.org/ .

Copyright © 1995.

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