Title

Induction of bone morphogenetic protein-2 by interleukin-1 in human fibroblasts

Document Type

Article

Publication Date

1998

Abstract

Rheumatoid arthritis and periodontitis are chronic inflammatory diseases associated with tissue destruction that is mediated in part by elevated levels of cytokines (e.g. interleukin-1 and tumor necrosis factor). Differential screening of a human synovial fibroblast cDNA library for interleukin-1 induced genes revealed a clone identical to the gene encoding human bone morphogenetic protein-2. Northern blot analysis of human synovial fibroblast mRNA confirmed up-regulation of bone morphogenetic protein-2 in the presence of interleukin-1. Utilizing a specific antibody, levels of bone morphogenetic protein-2 protein in conditioned medium from synovial fibroblasts were also up-regulated in the presence of interleukin-1. This is the first report of the production of bone morphogenetic protein-2 by synovial fibroblasts, and the first report of its up-regulation in response to interleukin-1. However, interleukin-1 did not induce bone morphogenetic protein-2 mRNA in human gingival fibroblasts.

Publication Title

Biochemical and biophysical research communications

Volume

248

Issue

3

First Page

450

Last Page

453

Comments

This article was published in Biochemical and biophysical research communications, Volume 248, Issue 3, Pages 450-453.

The published version is available at http://dx.doi.org/10.1006/bbrc.1998.8988.

Copyright © 1998 Elsevier.

This document is currently not available here.

COinS