Title

NF-kB binds to a polymorphic repressor element in the MMP-3 promoter

Document Type

Article

Publication Date

2004

Abstract

A 5T/6T polymorphic site in the matrix metalloproteinase-3 (MMP-3) promoter has been identified as a repressor element involved in inhibiting induction of MMP-3 transcription by interleukin 1; and the 6T allele has been associated with decreased expression of MMP-3 as compared to the 5T allele. Zinc-binding protein-89 (ZBP-89) was cloned from a yeast one-hybrid assay via its ability to interact with this site, but when the protein was over-expressed, it resulted in activation of the MMP-3 promoter rather than repression. Here we show that in nuclear extracts isolated from human gingival fibroblasts stimulated with IL-1, this site is bound by p50 and p65 components of NF-kB in addition to ZBP-89, and that recombinant p50 binds preferentially to the 6T binding site. These results are consistent with a role for NF-kB in limiting the cytokine induced expression of MMP-3.

Publication Title

Biochemical and biophysical research communications

Volume

316

Issue

1

First Page

182

Last Page

188

Comments

This article was published in Biochemical and biophysical research communications, Volume 316, Issue 1, Pages 182-188.

The published version is available at http://dx.doi.org/10.1016/j.bbrc.2004.02.030.

Copyright © 2004 Elsevier.

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