Title

Phosphorylation of zipcode binding protein 1 is required for brain-derived neurotrophic factor signaling of local ß-actin synthesis and growth cone turning

Document Type

Article

Publication Date

2010

Abstract

The localization of specific mRNAs and their local translation in growth cones of developing axons has been shown to play an important mechanism to regulate growth cone turning responses to attractive or repulsive cues. However, the mechanism whereby local translation and growth cone turning may be controlled by specific mRNA-binding proteins is unknown. Here we demonstrate that brain-derived neurotrophic factor (BDNF) signals the Src-dependent phosphorylation of the ß-actinmRNAzipcode binding protein 1 (ZBP1), which is necessary for ß-actin synthesis and growth cone turning. We raised a phospho-specific ZBP1 antibody to Tyr396, which is a Src phosphorylation site, and immunofluorescence revealed BDNF-induced phosphorylation of ZBP1 within growth cones. The BDNF-induced increase in fluorescent signal of a green fluorescent protein translation reporter with the 3´ untranslated region of ß-actin was attenuated with the Src family kinase-specific inhibitor PP2 [4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine]. Furthermore, a nonphosphorylatable mutant, ZBP1 Y396F, suppressed the BDNF-induced and protein synthesis-dependent increase in ß-actin localization in growth cones. Last, the ZBP1 Y396F mutant blocked BDNF-induced attractive growth cone turning. These results indicate that phosphorylation of ZBP1 at Tyr396 within growth cones has a critical role to regulate local protein synthesis and growth cone turning. Our findings provide new insight into how the regulated phosphorylation of mRNA-binding proteins influences local translation underlying growth cone motility and axon guidance.

Publication Title

Journal of Neuroscience

Volume

30

Issue

28

First Page

9349

Last Page

9358

Comments

This article was published in Journal of Neuroscience, Volume 30, Issue 28, Pages 9349-9358.

The published version is available at http://dx.doi.org/10.1523/JNEUROSCI.0499-10.2010.

Copyright © 2010 the authors.

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